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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC12A9 Polyclonal antibody (26827-1-AP)

    WB analysis using 26827-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26827-1-AP (SLC12A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC12A9 Polyclonal antibody (26827-1-AP)

    WB analysis using 26827-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26827-1-AP (SLC12A9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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SLC12A9 Polyclonal antibody

SLC12A9 Polyclonal Antibody for WB, ELISA
Cat No. 26827-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CCC interacting protein 1, CCC6, CIP1, hCCC6, hCIP1, SLC12A9, WO3.3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, LNCaP cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26827-1-AP targets SLC12A9 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC12A9 fusion protein Ag25351 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium/chloride transporters), member 9
Calculated molecular weight 96 kDa
Observed molecular weight101 kDa
GenBank accession numberBC000154
Gene Symbol SLC12A9
Gene ID (NCBI) 56996
RRIDAB_3085908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC12A9, also known as CCC6 and CIP1, belongs to the SLC12A transporter family. SLC12A9 encodes a 914 amino acid protein with predicted 12 TM domains, two N-glycosylation sites between TM5 and TM6, and a carboxyl terminus PKC phosphorylation site (PMID: 23506871). SLC12A9 regulates ion gradients across renal tubules and maintains cell volume (PMID: 27530302). SLC12A9 is highly expressed in placenta, brain, and kidney.

Protocols

Product Specific Protocols
WB protocol for SLC12A9 antibody 26827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols