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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC13A3 Polyclonal antibody

SLC13A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26182-1-AP

Synonyms

hNaDC3, NaDC 3, NADC3, SDCT2, SLC13A3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using SLC13A3 Polyclonal antibody (26182-1-AP)

    WB analysis of mouse kidney using 26182-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26182-1-AP (SLC13A3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC13A3 Polyclonal antibody (26182-1-AP)

    WB analysis of HEK-293 using 26182-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26182-1-AP (SLC13A3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26182-1-AP targets SLC13A3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC13A3 fusion protein Ag24183 相同性解析による交差性が予測される生物種
Full Name solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
Calculated molecular weight 602 aa, 67 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC035966
Gene symbol SLC13A3
Gene ID (NCBI) 64849
RRIDAB_2880414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC13A3, also named NADC3 and SDCT2, belongs to the SLC13A/DASS transporter (TC 2.A.47) family and NADC subfamily. SLC13A3 encodes the plasma membrane Na+ /Dicarboxylate Cotransporter 3 (NaDC3), which imports inside the cell four to six carbon dicarboxylates as well as N-acetylaspartate (NAA). SLC13A3 is mainly expressed in kidney, but also in brain, liver, placenta and eye (PMID: 30635937). This antibody detected the 55-60 kDa band in SDS-PAGE. However, the 55 kDa (nonglycosylated) and 85 kDa (glycosylated) bands have been reported in the article (PMID: 27053689).

Protocols

Product Specific Protocols
WB protocol for SLC13A3 antibody 26182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Succinate Accumulation Is Associated with a Shift of Mitochondrial Respiratory Control and HIF-1α Upregulation in PTEN Negative Prostate Cancer Cells.

Authors - Anja Weber
View Article