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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC14A2 Polyclonal antibody (21932-1-AP)

    WB analysis using 21932-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21932-1-AP (SLC14A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC14A2 Polyclonal antibody (21932-1-AP)

    WB analysis using 21932-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21932-1-AP (SLC14A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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SLC14A2 Polyclonal antibody

SLC14A2 Polyclonal Antibody for WB, ELISA
Cat No. 21932-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

hUT A6, HUT2, SLC14A2, Urea transporter 2, Urea transporter, kidney, UT A2, UT2, UTA, UTR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21932-1-AP targets SLC14A2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC14A2 fusion protein Ag16561 相同性解析による交差性が予測される生物種
Full Name solute carrier family 14 (urea transporter), member 2
Calculated molecular weight 920 aa, 101 kDa
Observed molecular weight43 kDa, 101 kDa
GenBank accession numberBC110445
Gene Symbol SLC14A2
Gene ID (NCBI) 8170
RRIDAB_3085679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15849
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC14A2, also known as human urea transporter -2 (HUT2), is the human homolog of UT-A2 and the only kidney-specific urea transporter cloned in humans. Urea transport in the kidney is mediated by a family of transporter proteins, including renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). UT-A proteins expressed in the kidney play a critical role in generating the hypertonic medulla, which is an integral component of the urinary concentrating mechanism (PMID: 11590132, 11880324). The 4.2-kb hUT-A1 cDNA encodes a 920-amino acid peptide, which is 89% identical to the rat UT-A1 protein (PMID: 11502588).

Protocols

Product Specific Protocols
WB protocol for SLC14A2 antibody 21932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols