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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC15A1/PEPT1 Polyclonal antibody

SLC15A1/PEPT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21726-1-AP

Synonyms

HPECT1, HPEPT1, PEPT1, Peptide transporter 1, SLC15A1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SLC15A1/PEPT1 Polyclonal antibody (21726-1-AP)

    WB analysis using 21726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21726-1-AP (SLC15A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC15A1/PEPT1 Polyclonal antibody (21726-1-AP)

    WB analysis using 21726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21726-1-AP (SLC15A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCOLO 320 cells, Caco-2 cells, HT-29 cells, MOLT-4 cells, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

21726-1-AP targets SLC15A1/PEPT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC15A1/PEPT1 fusion protein Ag16390 相同性解析による交差性が予測される生物種
Full Name solute carrier family 15 (oligopeptide transporter), member 1
Calculated molecular weight 708 aa, 79 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC096327
Gene symbol SLC15A1/PEPT1
Gene ID (NCBI) 6564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC15A1/PEPT1 is a peptide transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. PEPT1 mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system. PEPT1 is primarily expressed in the small intestine (PMID: 22194420). PEPT1 is also expressed in distal colon in rodents and humans and contributes to water absorption. 71 kDa nonglycosylated PEPT1 protein can be detected (PMID: 23660505).

Protocols

Product Specific Protocols
WB protocol for SLC15A1/PEPT1 antibody 21726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Photodiagnosis Photodyn Ther

Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.

Authors - Kumi Ogihara
View Article