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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC16A11 Recombinant antibody (83239-5-RR)

    WB analysis using 83239-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83239-5-RR (SLC16A11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using SLC16A11 Recombinant antibody (83239-5-RR)

    FC experiment of A549 using 83239-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SLC16A11 Recombinant antibody (83239-5-RR, Clone:240081H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83239-5-RR

    Affinity and Kinetic Characterization of 83239-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83239-5-RR against Human SLC16A11 were performed. The affinity constant is below 1 pM.

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SLC16A11 Recombinant antibody

SLC16A11 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83239-5-RR
Clone No.240081H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Monocarboxylate transporter 11, MCT11, MCT 11, FLJ90193, 240081H5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, mouse brain tissue, mouse stomach tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83239-5-RR targets SLC16A11 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC16A11 fusion protein Ag24920 相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 11 (monocarboxylic acid transporter 11)
Calculated molecular weight 471 aa, 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC093860
Gene Symbol SLC16A11
Gene ID (NCBI) 162515
RRIDAB_3670915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NCK7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC16A11 antibody 83239-5-RRDownload protocol
FC protocol for SLC16A11 antibody 83239-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols