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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    WB analysis of mouse kidney using 20553-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    WB analysis of mouse kidney using 20553-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of human stomach cancer using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of human kidney using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of mouse kidney using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of human testis using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of human testis using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of mouse kidney using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC16A12 Polyclonal antibody (20553-1-AP)

    IHC staining of mouse kidney using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC16A12 Polyclonal antibody

SLC16A12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20553-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

Monocarboxylate transporter 12, MCT12, MCT 12, CRT2, Creatine transporter 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20553-1-AP targets SLC16A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 12 (monocarboxylic acid transporter 12)
Calculated molecular weight 53 kDa
Observed molecular weight53-56 kDa
GenBank accession numberNM_213606
Gene Symbol SLC16A12
Gene ID (NCBI) 387700
RRIDAB_10693621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZSM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.

Protocols

Product Specific Protocols
WB protocol for SLC16A12 antibody 20553-1-APDownload protocol
IHC protocol for SLC16A12 antibody 20553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
XenopusWB

Biochem Biophys Res Commun

Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter.

Authors - Yuya Futagi
View Article
humanIHC

Medicine (Baltimore)

Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma.

Authors - Jie Mei
View Article