Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of HeLa using 22787-1-AP
WB result of MCT4 antibody (22787-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT4 transfected HeLa cells.
WB analysis using 22787-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 22787-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 22787-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 22787-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 22787-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
IF Staining of HepG2 using 22787-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 22787-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
FC experiment of HepG2 using 22787-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human MCT4 (22787-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

MCT4 Polyclonal antibody

MCT4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 22787-1-AP

Publications(82)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

SLC16A3, MCT-4, MCT3, MCT 4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (82)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, rat skeletal muscle tissue, HepG2 cells, PC-3 cells
Positive IHC detected in	human breast cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, mouse skeletal muscle tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:20000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 61 publications below
IHC	See 10 publications below
IF	See 13 publications below
IP	See 1 publications below
FC	See 3 publications below

Product Information

22787-1-AP targets MCT4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCT4 fusion protein Ag18788 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
Calculated molecular weight	465 aa, 49 kDa
Observed molecular weight	38-42 kDa
GenBank accession number	BC112269
Gene Symbol	MCT4
Gene ID (NCBI)	9123
RRID	AB_11182479
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15427
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

Protocols

Product Specific Protocols
WB protocol for MCT4 antibody 22787-1-AP	Download protocol
IHC protocol for MCT4 antibody 22787-1-AP	Download protocol
IF protocol for MCT4 antibody 22787-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
mouse	WB	Nat Cancer Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer Authors - Federica Cappellesso View Article
human	WB	Nat Metab Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma Authors - Zijian Yang KD Validated View Article
		Adv Sci (Weinh) SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle Authors - Xiaowei She View Article
mouse	IF	Nat Commun Lactate sensing mechanisms in arterial chemoreceptor cells. Authors - Hortensia Torres-Torrelo View Article
mouse	WB	Adv Healthc Mater Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature. Authors - Zhijie Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 22787-1-AP

WB analysis using 22787-1-AP

WB analysis using 22787-1-AP

WB analysis of rat skeletal muscle using 22787-1-AP

IHC staining of human prostate cancer using 22787-1-AP

IHC staining of human prostate cancer using 22787-1-AP

IHC staining of human renal cell carcinoma using 22787-1-AP

IHC staining of mouse skeletal muscle using 22787-1-AP

IHC staining of human breast cancer using 22787-1-AP

IHC staining of human breast cancer using 22787-1-AP

IHC staining of human breast cancer using 22787-1-AP

IHC staining of human skeletal muscle using 22787-1-AP

IHC staining of human skeletal muscle using 22787-1-AP

IHC staining of human prostate cancer using 22787-1-AP

IHC staining of human prostate cancer using 22787-1-AP

IF Staining of HepG2 using 22787-1-AP

IF Staining of HepG2 using 22787-1-AP

IF Staining of HepG2 using 22787-1-AP

FC experiment of HepG2 using 22787-1-AP

MCT4 Polyclonal antibody

MCT4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle

Lactate sensing mechanisms in arterial chemoreceptor cells.

Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature.