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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC16A4 Polyclonal antibody

SLC16A4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20889-1-AP

Synonyms

MCT 4, MCT 5, MCT4, MCT5, Monocarboxylate transporter 4, Monocarboxylate transporter 5, SLC16A4

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SLC16A4 Polyclonal antibody (20889-1-AP)

    WB analysis of Jurkat using 20889-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20889-1-AP (SLC16A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC16A4 Polyclonal antibody (20889-1-AP)

    WB analysis using 20889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20889-1-AP (SLC16A4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC16A4 Polyclonal antibody (20889-1-AP)

    IHC staining of human stomach cancer using 20889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC16A4 Polyclonal antibody (20889-1-AP)

    IHC staining of human stomach cancer using 20889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC16A4 Polyclonal antibody (20889-1-AP)

    IHC staining of human stomach cancer using 20889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC16A4 Polyclonal antibody (20889-1-AP)

    IHC staining of human stomach cancer using 20889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20889-1-AP targets SLC16A4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC16A4 fusion protein Ag14190 相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 4 (monocarboxylic acid transporter 5)
Calculated molecular weight 487 aa, 54 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC021664
Gene symbol SLC16A4
Gene ID (NCBI) 9122
RRIDAB_2878757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC16A4, also named as MCT5, is a proton-linked monocarboxylate transporter which can catalyze the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A4 is expressed in human intestine, liver and kidney. (PMID:18996437)

Protocols

Product Specific Protocols
WB protocol for SLC16A4 antibody 20889-1-APDownload protocol
IHC protocol for SLC16A4 antibody 20889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Arq Bras Cardiol

SLC26A4-AS1 Aggravates AngII-induced Cardiac Hypertrophy by Enhancing SLC26A4 Expression

Authors - Xiaoliang Han
View Article
humanWB

Int J Biochem Cell Biol

The CDK4/6 inhibitor PD0332991 stabilizes FBP1 by repressing MAGED1 expression in pancreatic ductal adenocarcinoma.

Authors - Bin Zhang
View Article