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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC16A5 Polyclonal antibody

SLC16A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12120-1-AP

Synonyms

MCT 5, MCT 6, MCT5, MCT6, Monocarboxylate transporter 5, Monocarboxylate transporter 6, SLC16A5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    WB analysis of mouse skeletal muscle using 12120-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    WB analysis of human placenta using 12120-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    IHC staining of human breast cancer using 12120-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    IHC staining of human kidney using 12120-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC16A5 Polyclonal antibody (12120-1-AP)

    IHC staining of human kidney using 12120-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human placenta tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12120-1-AP targets SLC16A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC16A5 fusion protein Ag2762 相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 5 (monocarboxylic acid transporter 6)
Calculated molecular weight 505 aa, 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC009684
Gene symbol SLC16A5
Gene ID (NCBI) 9121
RRIDAB_2877827
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Monocarboxylate transporter 5 (SLC16A5), also named as MCT6, is a member of the MCT family. SLC16A5 is a proton-linked monocarboxylate transporter that catalyzes the rapid transport across the plasma membrane of many monocarboxylates and it can transport various drugs. It has been reported that SLC16A5 mutation may be a novel genetic risk factor for cisplatin-induced ototoxic (CIO) in testicular cancer patients. There are some isoforms of SLC16A5 among 50-55 kDa. (PMID: 16174808, 28448657)

Protocols

Product Specific Protocols
WB protocol for SLC16A5 antibody 12120-1-APDownload protocol
IHC protocol for SLC16A5 antibody 12120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols