Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
20751-1-AP targets NPT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NPT1 fusion protein Ag12819 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 17 (sodium phosphate), member 1 |
| Calculated molecular weight | 467 aa, 51 kDa |
| Observed molecular weight | 50 kDa, 60-64 kDa |
| GenBank accession number | BC101745 |
| Gene Symbol | NPT1 |
| Gene ID (NCBI) | 6568 |
| RRID | AB_10693677 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPT1 (also known as SLC17A1) is a member of sodium-dependent inorganic phosphate transporters (NPT), which regulates entrance into the cellular membrane. NPT1 is mainly expressed in the kidney transporting small organic anions such as PAH (para-aminohippurate), but it is also found in the liver and brain. NTP1 localizes to the apical membrane of renal proximal tubular cells. NPT1 exits two isoforms due to the alternative splicing. Western blot analysis using this antibody detected a major band around 45-55 kDa in kidney tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPT1 antibody 20751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Mol Med Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury. | ||
Phytomedicine Eurycomanol alleviates hyperuricemia by promoting uric acid excretion and reducing purine synthesis. | ||
Front Pharmacol Effect of Eurycoma longifolia Stem Extract on Uric Acid Excretion in Hyperuricemia Mice. |