SLC17A9 Recombinant antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 83505-1-RR (SLC17A9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC17A9 Recombinant antibody (83505-1-RR, Clone:240328H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 83505-1-RR against Human SLC17A9 were performed. The affinity constant is below 1 pM.