Validation Data Gallery

Filter: AllWBIP

WB analysis using 13407-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13407-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 13407-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 13407-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13407-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 13407-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 13407-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 13407-1-AP
IP result of anti-SLC19A3 (IP:13407-1-AP, 4ug; Detection:13407-1-AP 1:600) with mouse liver tissue lysate 4000ug.

SLC19A3 Polyclonal antibody

SLC19A3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13407-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Synonyms

SLC19A3, Thiamine transporter 2, ThTr 2, THTR2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse kidney tissue, human placenta tissue, rat heart tissue, 3T3-L1 cells, rat liver tissue
Positive IP detected in	mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IF	See 1 publications below

Product Information

13407-1-AP targets SLC19A3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC19A3 fusion protein Ag4053 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 19, member 3
Calculated molecular weight	496 aa, 56 kDa
Observed molecular weight	63-70 kDa
GenBank accession number	BC032014
Gene Symbol	SLC19A3
Gene ID (NCBI)	80704
RRID	AB_2187997
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZV2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC19A3 antibody 13407-1-AP	Download protocol
IP protocol for SLC19A3 antibody 13407-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Sci Transl Med CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease. Authors - Sara Picó View Article
human	WB	Nat Commun Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia Authors - Alaullah Sheikh View Article
human	WB	Front Cell Dev Biol Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress. Authors - Jane L Tarry-Adkins View Article
human	WB,IF	J Nutr Biochem Up-regulation of vitamin B1 homeostasis genes in breast cancer. Authors - Zastre Jason A JA View Article
human	WB	J Biol Chem Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters. Authors - Subrata Sabui View Article
mouse	WB	Front Pharmacol Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice. Authors - Hong Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13407-1-AP

WB analysis using 13407-1-AP

WB analysis of SH-SY5Y using 13407-1-AP

WB analysis of mouse skeletal muscle using 13407-1-AP

WB analysis of mouse kidney using 13407-1-AP

WB analysis of human placenta using 13407-1-AP

WB analysis of rat heart using 13407-1-AP

IP experiment of mouse liver using 13407-1-AP

SLC19A3 Polyclonal antibody

SLC19A3 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.

Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia

Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.

Up-regulation of vitamin B1 homeostasis genes in breast cancer.

Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.

Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice.