SLC1A4 Polyclonal antibody

Name: SLC1A4 Polyclonal antibody
Brand: Proteintech
SKU: 13067-2-AP

SLC1A4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13067-2-AP

Synonyms

ASCT 1, ASCT1, SATT, SLC1A4

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 13067-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 13067-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 13067-2-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13067-2-AP
IP result of anti-SLC1A4 (IP:13067-2-AP, 4ug; Detection:13067-2-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of mouse kidney using 13067-2-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13067-2-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13067-2-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13067-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

-/ -

Cat No. 13067-2-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Raji cells, mouse brain tissue, human skeletal muscle tissue, SW480 cells, mouse kidney tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse kidney tissue, human kidney tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

13067-2-AP targets SLC1A4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC1A4 fusion protein Ag3763 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Calculated molecular weight	532 aa, 56 kDa
Observed molecular weight	62-70 kDa
GenBank accession number	BC026216
Gene symbol	SLC1A4
Gene ID (NCBI)	6509
RRID	AB_2190604
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC1A4 antibody 13067-2-AP	Download protocol
IHC protocol for SLC1A4 antibody 13067-2-AP	Download protocol
IP protocol for SLC1A4 antibody 13067-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner Authors - Xin Liu View Article
mouse	WB	Ecotoxicol Environ Saf Toxic effects of maternal cadmium exposure on the metabolism and transport system of amino acids in the maternal livers Authors - Peng Xu View Article
human	WB	Front Oncol SLC1A4: A Powerful Prognostic Marker and Promising Therapeutic Target for HCC. Authors - Xiaozhen Peng View Article
mouse	WB	Res Sq Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM) Authors - Megan L Ratz View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC1A4 Polyclonal antibody

SLC1A4 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 13067-2-AP

WB analysis using 13067-2-AP

WB analysis of human skeletal muscle using 13067-2-AP

IP experiment of mouse brain using 13067-2-AP

IHC staining of mouse kidney using 13067-2-AP

IHC staining of human kidney using 13067-2-AP

IHC staining of mouse kidney using 13067-2-AP

IHC staining of mouse brain using 13067-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner

Toxic effects of maternal cadmium exposure on the metabolism and transport system of amino acids in the maternal livers

SLC1A4: A Powerful Prognostic Marker and Promising Therapeutic Target for HCC.

Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM)