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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    WB analysis using 68540-1-Ig (same clone as 68540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68540-1-Ig (SLC1A5/ASCT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    IHC staining of human stomach cancer using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    IHC staining of human stomach cancer using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    IF Staining of HEK-293 using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (68540-1-Ig, Clone: 1C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    FC experiment of HeLa using 68540-1-Ig (same clone as 68540-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (68540-1-Ig, Clone:1C4E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

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SLC1A5/ASCT2 Monoclonal antibody, PBS Only

SLC1A5/ASCT2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68540-1-PBS
Clone No.1C4E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SLC1A5, Baboon M7 virus receptor, ATBO, ATB(0), ASCT2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68540-1-PBS targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC1A5/ASCT2 fusion protein Ag16837 相同性解析による交差性が予測される生物種
Full Name solute carrier family 1 (neutral amino acid transporter), member 5
Calculated molecular weight 541 aa, 57 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC000062
Gene Symbol SLC1A5/ASCT2
Gene ID (NCBI) 6510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15758
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).