Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HEK-293 using 12423-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12423-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12423-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12423-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12423-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12423-1-AP
IP result of anti-SLC20A1 (IP:12423-1-AP, 4ug; Detection:12423-1-AP 1:500) with HEK-293 cells lysate 2800ug.
IHC staining of human liver using 12423-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12423-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12423-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12423-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SLC20A1 Polyclonal antibody

SLC20A1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12423-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

PIT1, Phosphate transporter 1, Leukemia virus receptor 1 homolog, GLVR-1, GLVR1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, human kidney tissue, Jurkat cells, K-562 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver tissue, human breast cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below
FC	See 1 publications below
CoIP	See 1 publications below

Product Information

12423-1-AP targets SLC20A1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC20A1 fusion protein Ag3084 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 20 (phosphate transporter), member 1
Calculated molecular weight	679 aa, 74 kDa
Observed molecular weight	85 kDa
GenBank accession number	BC019944
Gene Symbol	SLC20A1
Gene ID (NCBI)	6574
RRID	AB_2286212
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WUM9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.

Protocols

Product Specific Protocols
WB protocol for SLC20A1 antibody 12423-1-AP	Download protocol
IHC protocol for SLC20A1 antibody 12423-1-AP	Download protocol
IP protocol for SLC20A1 antibody 12423-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	FC	Nat Cancer GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity Authors - Dionysios C Watson View Article
human,mouse	WB,CoIP	Theranostics SGK3 promotes vascular calcification via Pit-1 in chronic kidney disease Authors - Qing-Qing Dong View Article
human	WB	Adv Healthc Mater Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds Authors - Wei Chen KD Validated View Article
human	WB	FASEB J PMID: 27565711 Authors - Tuyet Thi Nguyen View Article
zebrafish	IF	Front Cell Dev Biol SLC20A1 Is Involved in Urinary Tract and Urorectal Development. Authors - Johanna Magdalena Rieke View Article
human	WB	J Biol Chem Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake. Authors - Nina Bon View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 12423-1-AP

WB analysis of HeLa using 12423-1-AP

WB analysis of human kidney using 12423-1-AP

WB analysis of K-562 using 12423-1-AP

WB analysis of Jurkat using 12423-1-AP

IP experiment of HEK-293 using 12423-1-AP

IHC staining of human liver using 12423-1-AP

IHC staining of human liver using 12423-1-AP

IHC staining of human breast cancer using 12423-1-AP

IHC staining of human breast cancer using 12423-1-AP

IHC staining of human lymphoma using 12423-1-AP

IHC staining of human lymphoma using 12423-1-AP

SLC20A1 Polyclonal antibody

SLC20A1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity

SGK3 promotes vascular calcification via Pit-1 in chronic kidney disease

Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds

PMID: 27565711

SLC20A1 Is Involved in Urinary Tract and Urorectal Development.

Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake.