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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC20A2 Polyclonal antibody (12820-1-AP)

    WB analysis using 12820-1-AP (same clone as 12820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using SLC20A2 Polyclonal antibody (12820-1-AP)

    WB analysis of COLO 320 using 12820-1-AP (same clone as 12820-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SLC20A2 Polyclonal antibody (12820-1-AP)

    IP experiment of COLO 320 using 12820-1-AP (same clone as 12820-1-PBS)

    IP result of anti-SLC20A2 (IP:12820-1-AP, 4ug; Detection:12820-1-AP 1:400) with COLO 320 cells lysate 2400ug. This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using SLC20A2 Polyclonal antibody (12820-1-AP)

    IHC staining of human bowen disease using 12820-1-AP (same clone as 12820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using SLC20A2 Polyclonal antibody (12820-1-AP)

    IHC staining of human bowen disease using 12820-1-AP (same clone as 12820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC20A2 Polyclonal antibody (12820-1-AP)

    IHC staining of mouse testis using 12820-1-AP (same clone as 12820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC20A2 Polyclonal antibody (12820-1-AP)

    IHC staining of mouse testis using 12820-1-AP (same clone as 12820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC20A2 Polyclonal antibody (12820-1-AP)

    IF Staining of mouse brain using 12820-1-AP (same clone as 12820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC20A2 antibody (12820-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12820-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SLC20A2 Polyclonal antibody, PBS Only

SLC20A2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 12820-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Pit2, Phosphate transporter 2, hPit2, GLVR-2, GLVR2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12820-1-PBS targets SLC20A2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC20A2 fusion protein Ag3469 相同性解析による交差性が予測される生物種
Full Name solute carrier family 20 (phosphate transporter), member 2
Calculated molecular weight 652 aa, 70 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC028600
Gene Symbol SLC20A2
Gene ID (NCBI) 6575
RRIDAB_2191004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08357
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment.