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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC22A16 Polyclonal antibody

SLC22A16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26833-1-AP

Synonyms

Carnitine transporter 2, CT2, Flipt 2, FLIPT2, OCT6, OKB1, SLC22A16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC22A16 Polyclonal antibody (26833-1-AP)

    WB analysis using 26833-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26833-1-AP (SLC22A16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC22A16 Polyclonal antibody (26833-1-AP)

    IHC staining of human breast cancer using 26833-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC22A16 Polyclonal antibody (26833-1-AP)

    IHC staining of human stomach cancer using 26833-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC22A16 Polyclonal antibody (26833-1-AP)

    IHC staining of human stomach cancer using 26833-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC22A16 Polyclonal antibody (26833-1-AP)

    IHC staining of human breast cancer using 26833-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using SLC22A16 Polyclonal antibody (26833-1-AP)

    IF Staining of THP-1 using 26833-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SLC22A16 antibody (26833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26833-1-AP targets SLC22A16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC22A16 fusion protein Ag25270 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic cation/carnitine transporter), member 16
Calculated molecular weight 65 kDa
Observed molecular weight68 kDa
GenBank accession numberBC047565
Gene symbol SLC22A16
Gene ID (NCBI) 85413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC22A16, also named as CT2, FLIPT2, or OCT6, belongs to the major facilitator (TC 2.A.1) superfamily and organic cation transporter (TC 2.A.1.19) family. SLC22A16 encodes an organic zwitterion transporter protein that transports carnitine and some anticancer drugs, including anthracyclines. As a carnitine transporter, SLC22A16 is involved in the regulation of fatty acid oxidation (FAO), which plays an important role also in the metabolic transformation of cancer cells. SLC22A16 expression in cancer cells is associated with increased sensitivity to the cytotoxic effects of doxorubicin. SLC22A16 has 3 isoforms with the molecular mass of 58, 61 and 65 kDa (PMID: 35761855, 35608746).

Protocols

Product Specific Protocols
WB protocol for SLC22A16 antibody 26833-1-APDownload protocol
IHC protocol for SLC22A16 antibody 26833-1-APDownload protocol
IF protocol for SLC22A16 antibody 26833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols