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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of L02 using 24901-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of mouse liver using 24901-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of rat liver using 24901-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of mouse kidney using 24901-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    IP experiment of mouse liver using 24901-1-AP

    IP result of anti-SLC22A23 (IP:24901-1-AP, 4ug; Detection:24901-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC22A23 Polyclonal antibody

SLC22A23 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24901-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

BOCT2, C6orf85, SLC22A23

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24901-1-AP targets SLC22A23 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC22A23 fusion protein Ag19036 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22, member 23
Calculated molecular weight 686 aa, 74 kDa
Observed molecular weight 66-74 kDa
GenBank accession numberBC128580
Gene Symbol SLC22A23
Gene ID (NCBI) 63027
RRIDAB_2879787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA1A5C7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC22A23 antibody 24901-1-APDownload protocol
IHC protocol for SLC22A23 antibody 24901-1-APDownload protocol
IP protocol for SLC22A23 antibody 24901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols