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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC22A23 Polyclonal antibody

SLC22A23 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24901-1-AP

Synonyms

BOCT2, C6orf85, SLC22A23

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of L02 using 24901-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of mouse liver using 24901-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of rat liver using 24901-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    WB analysis of mouse kidney using 24901-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    IP experiment of mouse liver using 24901-1-AP

    IP result of anti-SLC22A23 (IP:24901-1-AP, 4ug; Detection:24901-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC22A23 Polyclonal antibody (24901-1-AP)

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24901-1-AP is SLC22A23 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC22A23 fusion protein Ag19036 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22, member 23
Calculated molecular weight 686 aa, 74 kDa
Observed molecular weight 66-74 kDa
GenBank accession numberBC128580
Gene symbol SLC22A23
Gene ID (NCBI) 63027
RRIDAB_2879787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC22A23 antibody 24901-1-APDownload protocol
IHC protocol for SLC22A23 antibody 24901-1-APDownload protocol
IP protocol for SLC22A23 antibody 24901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols