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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC22A4 Polyclonal antibody (13238-1-AP)

    WB analysis using 13238-1-AP

    boiled and 37°C incubated mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 13238-1-AP (SLC22A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SLC22A4 Polyclonal antibody

SLC22A4 Polyclonal Antibody for WB, ELISA
Cat No. 13238-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Ergothioneine transporter, ET transporter, ETT, OCTN1, SLC22A4, UT2H

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse kidney tissue, 37°C incubated mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13238-1-AP targets SLC22A4 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC22A4 fusion protein Ag3974 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic cation/ergothioneine transporter), member 4
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight62-70 kDa
GenBank accession numberBC028313
Gene Symbol SLC22A4
Gene ID (NCBI) 6583
RRIDAB_3669155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H015
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC22A4 (solute carrier family 22 member 4), also known as OCTN1. It is expected to be located in cell membrane and mitochondrion membrane. It is strongly expressed in kidney, trachea, bone marrow and fetal liver and in several human cancer cell lines, but not in adult liver (PMID: 9426230). The protein functions as a Na+-dependent and pH-dependent high affinity microbial symporter of potent food-derived antioxidant ergothioeine (PubMed:15795384), which transports one sodium ion with one ergothioeine molecule. The calculated molecular weight of SLC22A4 is 62 kDa, the band of 70 kDa is glycosylated (PMID:26724204).

Protocols

Product Specific Protocols
WB protocol for SLC22A4 antibody 13238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols