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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC22A4 Recombinant antibody (83192-1-RR)

    WB analysis using 83192-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83192-1-RR (SLC22A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83192-1-RR

    Affinity and Kinetic Characterization of 83192-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83192-1-RR against Human SLC22A4 were performed. The affinity constant is 1.08 nM.

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SLC22A4 Recombinant antibody

SLC22A4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83192-1-RR
Clone No.240064D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

UT2H, OCTN1, ETTh, ETT, 240064D5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83192-1-RR targets SLC22A4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC22A4 fusion protein Ag3974 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic cation/ergothioneine transporter), member 4
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 62-65 kDa
GenBank accession numberBC028313
Gene Symbol SLC22A4
Gene ID (NCBI) 6583
RRIDAB_3670882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H015
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC22A4 (solute carrier family 22 member 4), also known as OCTN1. It is expected to be located in cell membrane and mitochondrion membrane. It is strongly expressed in kidney, trachea, bone marrow and fetal liver and in several human cancer cell lines, but not in adult liver (PMID: 9426230). The protein functions as a Na+-dependent and pH-dependent high affinity microbial symporter of potent food-derived antioxidant ergothioeine (PubMed:15795384), which transports one sodium ion with one ergothioeine molecule.

Protocols

Product Specific Protocols
WB protocol for SLC22A4 antibody 83192-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols