SLC22A7 Polyclonal antibody

Name: SLC22A7 Polyclonal antibody
Brand: Proteintech
SKU: 26796-1-AP

SLC22A7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26796-1-AP

Synonyms

hOAT2, NLT, Novel liver transporter, OAT2, Organic anion transporter 2, SLC22A7

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse kidney using 26796-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 26796-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 26796-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 26796-1-AP
IP result of anti-SLC22A7 (IP:26796-1-AP, 4ug; Detection:26796-1-AP 1:1000) with mouse liver tissue lysate 5200ug.
IHC staining of human liver using 26796-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 26796-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 26796-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 26796-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse kidney tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

The immunogen of 26796-1-AP is SLC22A7 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC22A7 fusion protein Ag25217 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 22 (organic anion transporter), member 7
Calculated molecular weight	60 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC033805
Gene symbol	SLC22A7
Gene ID (NCBI)	10864
RRID	AB_2880639
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC22A7 antibody 26796-1-AP	Download protocol
IHC protocol for SLC22A7 antibody 26796-1-AP	Download protocol
IP protocol for SLC22A7 antibody 26796-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biochem Pharmacol Upregulation of histone acetylation reverses organic anion transporter 2 repression and enhances 5-fluorouracil sensitivity in hepatocellular carcinoma. Authors - Yingying Wang View Article
mouse	WB	Nutrients Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent. Authors - Karolin Weitkunat View Article
human	WB	Drug Metab Pharmacokinet Inhibiting uptake activity of organic anion transporter 2 by bile acids. Authors - Yu Wang View Article
mouse	WB	Xenobiotica Changes in the expression of drug-metabolising enzymes and drug transporters in mice with collagen antibody-induced arthritis Authors - Chieri Fujino View Article
mouse		Am J Physiol Renal Physiol Tubular Dysfunction Impairs Renal Excretion of Pseudouridine in Diabetic Kidney Disease Authors - Anna V Mathew View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC22A7 Polyclonal antibody

SLC22A7 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse kidney using 26796-1-AP

WB analysis of HEK-293 using 26796-1-AP

WB analysis of rat liver using 26796-1-AP

IP experiment of mouse liver using 26796-1-AP

IHC staining of human liver using 26796-1-AP

IHC staining of human liver using 26796-1-AP

IHC staining of mouse liver using 26796-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Upregulation of histone acetylation reverses organic anion transporter 2 repression and enhances 5-fluorouracil sensitivity in hepatocellular carcinoma.

Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.

Inhibiting uptake activity of organic anion transporter 2 by bile acids.

Changes in the expression of drug-metabolising enzymes and drug transporters in mice with collagen antibody-induced arthritis

Tubular Dysfunction Impairs Renal Excretion of Pseudouridine in Diabetic Kidney Disease