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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC22A7 Monoclonal antibody

SLC22A7 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D3C3

Cat no : 67479-1-Ig

Synonyms

SLC22A7, 3D3C3, hOAT2, NLT, OAT2

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using SLC22A7 Monoclonal antibody (67479-1-Ig)

    WB analysis of SMMC-7721 using 67479-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC22A7 Monoclonal antibody (67479-1-Ig)

    IHC staining of human liver cancer using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC22A7 Monoclonal antibody (67479-1-Ig)

    IHC staining of human liver cancer using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC22A7 Monoclonal antibody (67479-1-Ig)

    IHC staining of human liver using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC22A7 Monoclonal antibody (67479-1-Ig)

    IHC staining of mouse liver using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67479-1-Ig targets SLC22A7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SLC22A7 fusion protein Ag25234 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic anion transporter), member 7
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC033805
Gene symbol SLC22A7
Gene ID (NCBI) 10864
RRIDAB_2882706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Organic anion transporter (OAT)2 (SLC22A7) belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of numerous organic ions including xenobiotics and endogenous substrates (PMID: 9529348, 20190416). SLC22A7 was originally identified as a novel liver-specific transporter because of its predominant mRNA expression in the rat liver (PMID: 15900017, 25904762). Human SLC22A7 exhibits a robust transport function for a wide array of naturally occurring nucleobases, nucleosides, and nucleotides with a particular role for cGMP (PMID: 18216183).

Protocols

Product Specific Protocols
WB protocol for SLC22A7 antibody 67479-1-IgDownload protocol
IHC protocol for SLC22A7 antibody 67479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols