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SLC24A4 Polyclonal antibody

Name: SLC24A4 Polyclonal antibody
Brand: Proteintech
SKU: 18992-1-AP

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18992-1-AP

Synonyms

Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4

Validation Data Gallery

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WB analysis using 18992-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 18992-1-AP
WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.
WB analysis of mouse brain using 18992-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 18992-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 18992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse eye using 18992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 18992-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, mouse heart tissue
Positive IHC detected in	mouse brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below

Product Information

18992-1-AP targets SLC24A4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
Calculated molecular weight	69 kDa
Observed molecular weight	62-90 kDa
GenBank accession number	NM_153646
Gene symbol	SLC24A4
Gene ID (NCBI)	123041
RRID	AB_10596939
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

Protocols

Product Specific Protocols
WB protocol for SLC24A4 antibody 18992-1-AP	Download protocol
IHC protocol for SLC24A4 antibody 18992-1-AP	Download protocol
IF protocol for SLC24A4 antibody 18992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Bone Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis. Authors - Akane Inoue View Article
rat	WB	J Cell Physiol Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling. Authors - Lacruz Rodrigo S RS View Article
mouse	WB	Calcif Tissue Int Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice. Authors - A L J J Bronckers View Article
human	WB	PeerJ Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma Authors - Mingpeng Jin View Article
mouse	WB	Cells Tissues Organs Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts. Authors - Hu Ping P View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 18992-1-AP

WB analysis of HeLa cells using 18992-1-AP

WB analysis of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of mouse brain using 18992-1-AP

IHC staining of human heart using 18992-1-AP

IHC staining of human heart using 18992-1-AP

IF Staining of mouse eye using 18992-1-AP

IF Staining of mouse eye using 18992-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.

Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.

Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.

Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma

Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.