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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A1 Monoclonal antibody (66771-1-Ig)

    WB analysis using 66771-1-Ig (same clone as 66771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66771-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLC25A1 Monoclonal antibody (66771-1-Ig)

    WB analysis using 66771-1-Ig (same clone as 66771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66771-1-PBS in a different storage buffer formulation.

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SLC25A1 Monoclonal antibody, PBS Only

SLC25A1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66771-1-PBS
Clone No.1F11A3

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66771-1-PBS targets SLC25A1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen SLC25A1 fusion protein Ag7520 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC004980
Gene Symbol SLC25A1
Gene ID (NCBI) 6576
RRIDAB_2882117
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53007
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)