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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A12 Monoclonal antibody, PBS Only (Detector)

SLC25A12 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B10

Cat no : 67467-1-PBS

Synonyms

Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial, Aralar1, Aralar, Araceli hiperlarga, AGC1

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using SLC25A12 Monoclonal antibody (67467-1-Ig)

    WB analysis of LNCaP using 67467-1-Ig (same clone as 67467-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SLC25A12 Monoclonal antibody (67467-1-Ig)

    WB analysis of Jurkat using 67467-1-Ig (same clone as 67467-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using SLC25A12 Monoclonal antibody (67467-1-Ig)

    WB analysis of A431 using 67467-1-Ig (same clone as 67467-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Monoclonal antibody (67467-1-Ig)

    IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50650-2

    Cytometric bead array standard curve of MP50650-2

    Cytometric bead array standard curve of MP50650-2, SLC25A12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67467-4-PBS. Detection antibody: 67467-1-PBS. Standard:Ag13576. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67467-1-PBS targets SLC25A12 as part of a matched antibody pair:

MP50650-2: 67467-4-PBS capture and 67467-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC25A12 fusion protein Ag13576 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Calculated molecular weight 75 kDa, 63 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC016932
Gene symbol SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2882696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.