SLC25A18 Polyclonal antibody
SLC25A18 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 17348-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, mouse kidney tissue, mouse heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
17348-1-AP targets SLC25A18 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SLC25A18 fusion protein Ag11420 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 25 (mitochondrial carrier), member 18 |
| Calculated molecular weight | 315 aa, 34 kDa |
| Observed molecular weight | 32-35 kDa |
| GenBank accession number | BC031644 |
| Gene symbol | SLC25A18 |
| Gene ID (NCBI) | 83733 |
| RRID | AB_2189889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC25A18, also named as GC 2, belongs to the mitochondrial transporter family (SLC25). It is located in inner mitochondrial membrane which involved in the transport of glutamate either with H+ or in exchange for OH-. SLC25A18 is highly expressed in brain, testis, lung, kidney, liver, spleen and skeletal muscle. 17348-1-AP antibody detects the band around 32-35 kDa in SDS-PAGE. (PMID: 14598172)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC25A18 antibody 17348-1-AP | Download protocol |
| IHC protocol for SLC25A18 antibody 17348-1-AP | Download protocol |
| IP protocol for SLC25A18 antibody 17348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Cancer Res SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling. | ||
Mitochondrion Increased expression of SLC25A18 is associated with Alzheimer's disease and is involved in Aβ42-induced mitochondrial dysfunction and apoptosis in neuronal cells | ||
MedComm (2020) Nicotinamide mononucleotide supplementation rescues mitochondrial and energy metabolism functions and ameliorates inflammatory states in the ovaries of aging mice |
