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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A2 Polyclonal antibody

SLC25A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21764-1-AP

Synonyms

ORC2, ORNT2, SLC25A2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using SLC25A2 Polyclonal antibody (21764-1-AP)

    WB analysis of mouse kidney using 21764-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21764-1-AP (SLC25A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SLC25A2 Polyclonal antibody (21764-1-AP)

    WB analysis of mouse liver using 21764-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21764-1-AP (SLC25A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLC25A2 Polyclonal antibody (21764-1-AP)

    WB analysis of HeLa using 21764-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21764-1-AP (SLC25A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC25A2 Polyclonal antibody (21764-1-AP)

    IHC staining of human liver using 21764-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21764-1-AP (SLC25A2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC25A2 Polyclonal antibody (21764-1-AP)

    IHC staining of human liver using 21764-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21764-1-AP (SLC25A2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC25A2 Polyclonal antibody (21764-1-AP)

    IHC staining of human kidney using 21764-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21764-1-AP (SLC25A2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC25A2 Polyclonal antibody (21764-1-AP)

    IHC staining of human kidney using 21764-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21764-1-AP (SLC25A2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21764-1-AP is SLC25A2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A2 fusion protein Ag16360 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC100935
Gene symbol SLC25A2
Gene ID (NCBI) 83884
RRIDAB_10858388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A2 antibody 21764-1-APDownload protocol
IHC protocol for SLC25A2 antibody 21764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Effects of canagliflozin on growth and metabolic reprograming in hepatocellular carcinoma cells: Multi-omics analysis of metabolomics and absolute quantification proteomics (iMPAQT).

Authors - Dan Nakano
View Article