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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A22 Polyclonal antibody

SLC25A22 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25402-1-AP

Synonyms

NET44, Glutamate/H(+) symporter 1, GC-1, GC1, GC 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A22 Polyclonal antibody (25402-1-AP)

    WB analysis using 25402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC25A22 Polyclonal antibody (25402-1-AP)

    WB analysis using 25402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC25A22 Polyclonal antibody (25402-1-AP)

    WB analysis using 25402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of mouse brain using 25402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of mouse brain using 25402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human colon cancer using 25402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human liver cancer using 25402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human liver cancer using 25402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human colon cancer using 25402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IF Staining of mouse brain using 25402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC25A22 antibody (25402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IF Staining of mouse brain using 25402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25402-1-AP (SLC25A22 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using SLC25A22 Polyclonal antibody (25402-1-AP)

    IF Staining of COS-7 using 25402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COS-7 cells using 25402-1-AP (SLC25A22 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, Caco-2 cells, HT-29 cells, rat brain tissue, NCI-H1299 cells
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25402-1-AP targets SLC25A22 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A22 fusion protein Ag19958 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier: glutamate), member 22
Calculated molecular weight 323 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC023545
Gene symbol SLC25A22
Gene ID (NCBI) 79751
RRIDAB_2880060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)

Protocols

Product Specific Protocols
WB protocol for SLC25A22 antibody 25402-1-APDownload protocol
IHC protocol for SLC25A22 antibody 25402-1-APDownload protocol
IF protocol for SLC25A22 antibody 25402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Dual-process brain mitochondria isolation preserves function and clarifies protein composition.

Authors - Maria F Noterman
View Article