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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A24 Polyclonal antibody

SLC25A24 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14669-1-AP

Synonyms

SCAMC 1, Mitochondrial Ca(2+)-dependent solute carrier protein 1, Mitochondrial ATP-Mg/Pi carrier protein 1, Mitochondrial adenyl nucleotide antiporter SLC25A24, MCSC1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SLC25A24 Polyclonal antibody (14669-1-AP)

    WB analysis of HEK-293 using 14669-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14669-1-AP (SLC25A24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using SLC25A24 Polyclonal antibody (14669-1-AP)

    WB analysis of mouse heart using 14669-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14669-1-AP (SLC25A24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SLC25A24 Polyclonal antibody (14669-1-AP)

    WB analysis of human placenta using 14669-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14669-1-AP (SLC25A24 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC25A24 Polyclonal antibody (14669-1-AP)

    IHC staining of mouse testis using 14669-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14669-1-AP (SLC25A24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC25A24 Polyclonal antibody (14669-1-AP)

    IHC staining of mouse testis using 14669-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14669-1-AP (SLC25A24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SLC25A24 Polyclonal antibody (14669-1-AP)

    IF Staining of A431 using 14669-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 14669-1-AP (SLC25A24 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue, human placenta tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14669-1-AP targets SLC25A24 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A24 fusion protein Ag6334 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24
Calculated molecular weight 53 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC068561
Gene symbol SLC25A24
Gene ID (NCBI) 29957
RRIDAB_10637267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A24 antibody 14669-1-APDownload protocol
IHC protocol for SLC25A24 antibody 14669-1-APDownload protocol
IF protocol for SLC25A24 antibody 14669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols