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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A36 Polyclonal antibody

SLC25A36 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26379-1-AP

Synonyms

Solute carrier family 25 member 36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A36 Polyclonal antibody (26379-1-AP)

    WB analysis using 26379-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26379-1-AP (SLC25A36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC25A36 Polyclonal antibody (26379-1-AP)

    IF Staining of HepG2 using 26379-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC25A36 antibody (26379-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26379-1-AP targets SLC25A36 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A36 fusion protein Ag23894 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 36
Observed molecular weight32-34 kDa
GenBank accession numberBC014064
Gene symbol SLC25A36
Gene ID (NCBI) 55186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC25A36 is a pyrimidine nucleotide carrier that belongs to the solute carrier family 25 (SLC25). SLC25A36 transports cytosine and uracil (deoxy)nucleosides monophosphate, diphosphate, and triphosphate via uniporter and antiporter(PMID: 25320081). It plays an important role in maintaining mitochondrial biogenesis. Loss of SLC25A36 in mouse embryonic stem cells (mESCs) is associated with mtDNA loss and mitochondrial dysfunction(PMID: 31036718).

Protocols

Product Specific Protocols
WB protocol for SLC25A36 antibody 26379-1-APDownload protocol
IF protocol for SLC25A36 antibody 26379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols