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Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of TF-1 using 67593-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of human placenta using 67593-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of human spleen using 67593-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of K-562 using 67593-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of LNCaP using 67593-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of L02 using 67593-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of HepG2 using 67593-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Mitoferrin 1 Monoclonal antibody

Mitoferrin 1 Monoclonal Antibody for WB, ELISA
Cat No. 67593-1-Ig
Clone No.1B1B2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

HT015, MFRN, Mitoferrin 1, MSC, MSCP, PRO1278, PRO1584, PRO2217, SLC25A37

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, HepG2 cells, human spleen tissue, K-562 cells, L02 cells, LNCaP cells, TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67593-1-Ig targets Mitoferrin 1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mitoferrin 1 fusion protein Ag26216 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 37
Observed molecular weight 40 kDa
GenBank accession numberBC132799
Gene Symbol Mitoferrin 1
Gene ID (NCBI) 51312
RRIDAB_2882800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NYZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitoferrin (mfrn, SLC25A37), which is highly expressed in fetal and adult hematopoietic tissues, is a member of the vertebrate mitochondrial solute carrier family (SLC25) and is located on the mitochondrial inner membrane. It functions as an essential and high-affinity iron importer for the biosynthesis of mitochondrial heme and iron-sulfur cluster in vertebrate erythroblasts. Additionally, since it was identified as a major depressive disorder (MDD) risk gene, it is likely to be used as a potential biomarker of MDD diagnose.


Protocols

Product Specific Protocols
WB protocol for Mitoferrin 1 antibody 67593-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols