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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SLC25A46 Polyclonal antibody (27869-1-AP)

    WB analysis of mouse brain using 27869-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27869-1-AP (SLC25A46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using SLC25A46 Polyclonal antibody (27869-1-AP)

    IHC staining of human colon using 27869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27869-1-AP (SLC25A46 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using SLC25A46 Polyclonal antibody (27869-1-AP)

    IHC staining of human colon using 27869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27869-1-AP (SLC25A46 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using SLC25A46 Polyclonal antibody (27869-1-AP)

    IHC staining of human medulloblastoma using 27869-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 27869-1-AP (SLC25A46 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using SLC25A46 Polyclonal antibody (27869-1-AP)

    IHC staining of human medulloblastoma using 27869-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 27869-1-AP (SLC25A46 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using SLC25A46 Polyclonal antibody (27869-1-AP)

    IF Staining of NIH/3T3 using 27869-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 27869-1-AP (SLC25A46 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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SLC25A46 Polyclonal antibody

SLC25A46 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27869-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SLC25A46, TB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

This antibody is only recommended for WB detection of tissue samples.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27869-1-AP targets SLC25A46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A46 fusion protein Ag27293 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 46
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC017169
Gene Symbol SLC25A46
Gene ID (NCBI) 91137
RRIDAB_2880999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AG3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Solute carrier family 25 member 46 (SLC25A46), a highly conserved protein among various species including vertebrates, is a nuclear gene encoding mitochondrial carriers. SLC25A46 is involved in mitochondrial fission and the transport of lipids from the endoplasmic reticulum to mitochondria (PMID: 33277645). SLC25A46 has 3 isoforms with molecular weights of 30, 37 and 46 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for SLC25A46 antibody 27869-1-APDownload protocol
IHC protocol for SLC25A46 antibody 27869-1-APDownload protocol
IF protocol for SLC25A46 antibody 27869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols