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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A46 Recombinant antibody

SLC25A46 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240114G3

Cat no : 83360-2-RR

Synonyms

TB1, Mitochondrial outer membrane protein SLC25A46, 240114G3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A46 Recombinant antibody (83360-2-RR)

    WB analysis using 83360-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83360-2-RR (SLC25A46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Caco-2 cells using SLC25A46 Recombinant antibody (83360-2-RR)

    FC experiment of Caco-2 using 83360-2-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using SLC25A46 Recombinant antibody (83360-2-RR)

    FC experiment of HeLa using 83360-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83360-2-RR

    Affinity and Kinetic Characterization of 83360-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83360-2-RR against Human SLC25A46 were performed. The affinity constant is 0.879 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, HeLa cells, Jurkat cells, MDA-MB-231 cells, mouse brain tissue
Positive FC (Intra) detected inCaco-2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83360-2-RR is SLC25A46 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC25A46 fusion protein Ag27293 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 46
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC017169
Gene symbol SLC25A46
Gene ID (NCBI) 91137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Solute carrier family 25 member 46 (SLC25A46), a highly conserved protein among various species including vertebrates, is a nuclear gene encoding mitochondrial carriers. SLC25A46 is involved in mitochondrial fission and the transport of lipids from the endoplasmic reticulum to mitochondria (PMID: 33277645). SLC25A46 has 3 isoforms with molecular weights of 30, 37 and 46 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for SLC25A46 antibody 83360-2-RRDownload protocol
FC protocol for SLC25A46 antibody 83360-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols