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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A6 Polyclonal antibody (51031-1-AP)

    WB analysis using 51031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51031-1-AP (SLC25A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC25A6 Polyclonal antibody (51031-1-AP)

    IHC staining of human lung cancer using 51031-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC25A6 Polyclonal antibody (51031-1-AP)

    IHC staining of human lung cancer using 51031-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC25A6 Polyclonal antibody (51031-1-AP)

    IHC staining of mouse liver using 51031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC25A6 Polyclonal antibody (51031-1-AP)

    IHC staining of mouse liver using 51031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC25A6 Polyclonal antibody

SLC25A6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51031-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ANT 3, ANT 2, ADP/ATP translocase 3, N-terminally processed, ADP/ATP translocase 3, ADP,ATP carrier protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, K-562 cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

51031-1-AP targets SLC25A6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
Calculated molecular weight 33 kDa
Observed molecular weight30-33 kDa
GenBank accession numberBC031912
Gene Symbol SLC25A6
Gene ID (NCBI) 293
RRIDAB_2190370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12236
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).

Protocols

Product Specific Protocols
WB protocol for SLC25A6 antibody 51031-1-APDownload protocol
IHC protocol for SLC25A6 antibody 51031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Oncol

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.

Authors - Mohamed E M Saeed
View Article
humanWB

MethodsX

Complete solubilization of mammalian cells in lysates

Authors - Izabela Bednarska
View Article