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SLC25A6-Specific Polyclonal antibody

Name: SLC25A6-Specific Polyclonal antibody
Brand: Proteintech
SKU: 14841-1-AP

SLC25A6-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14841-1-AP

Synonyms

SLC25A6, ANT 3, ANT 2, ADP/ATP translocase 3, N-terminally processed, ADP/ATP translocase 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14841-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14841-1-AP
WB result of SLC25A6-Specific antibody (14841-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A6-Specific transfected HEK-293 cells.
WB analysis of HEK-293 using 14841-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14841-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14841-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14841-1-AP
IP result of anti-SLC25A6-Specific (IP:14841-1-AP, 4ug; Detection:14841-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human skeletal muscle using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 14841-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 14841-1-AP
Immunofluorescent analysis of HepG2 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IF Staining of MCF-7 using 14841-1-AP
Immunofluorescent analysis of MCF-7 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Cat No. 14841-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	L02 cells, HEK-293 cells, human liver tissue, human heart tissue, human placenta tissue, HEK-293T cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human skeletal muscle tissue, human heart tissue, human kidney tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 2 publications below

Product Information

14841-1-AP targets SLC25A6-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
Calculated molecular weight	33 kDa
Observed molecular weight	30-33 kDa
GenBank accession number	NM_001636
Gene symbol	SLC25A6
Gene ID (NCBI)	293
RRID	AB_2190371
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A6-Specific antibody 14841-1-AP	Download protocol
IHC protocol for SLC25A6-Specific antibody 14841-1-AP	Download protocol
IF protocol for SLC25A6-Specific antibody 14841-1-AP	Download protocol
IP protocol for SLC25A6-Specific antibody 14841-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Curr Biol Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos. Authors - Ridhima Gomkale View Article
human	WB,CoIP	Am J Cancer Res PTPMT1 regulates mitochondrial death through the SLC25A6-NDUFS2 axis in pancreatic cancer cells Authors - Peng-Peng Ding View Article
	WB	Hum Mol Genet Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy. Authors - David Pacheu-Grau View Article
human	WB,IF	Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer. Authors - Yi Hao View Article
human	WB	FEBS Lett TIM29 is a subunit of the human carrier translocase required for protein transport. Authors - Sylvie Callegari View Article
human	WB,IHC,CoIP	iScience MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells Authors - Xingzhao Ji View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A6-Specific Polyclonal antibody

SLC25A6-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14841-1-AP

WB analysis of HEK-293 using 14841-1-AP

WB analysis of HEK-293 using 14841-1-AP

WB analysis of human liver using 14841-1-AP

WB analysis of human heart using 14841-1-AP

IP experiment of mouse liver using 14841-1-AP

IHC staining of human skeletal muscle using 14841-1-AP

IHC staining of human skeletal muscle using 14841-1-AP

IHC staining of human kidney using 14841-1-AP

IHC staining of human kidney using 14841-1-AP

IHC staining of human liver using 14841-1-AP

IHC staining of human liver using 14841-1-AP

IHC staining of human heart using 14841-1-AP

IHC staining of human heart using 14841-1-AP

IF Staining of HepG2 using 14841-1-AP

IF Staining of MCF-7 using 14841-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.

PTPMT1 regulates mitochondrial death through the SLC25A6-NDUFS2 axis in pancreatic cancer cells

Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.

Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.

TIM29 is a subunit of the human carrier translocase required for protein transport.

MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells