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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A6-Specific Recombinant antibody (85368-1-RR)

    WB analysis using 85368-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85368-1-RR (SLC25A6-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SLC25A6-Specific Recombinant antibody (85368-1-RR)

    IF Staining of A549 using 85368-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC25A6-Specific antibody (85368-1-RR, Clone: 242652A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SLC25A6-Specific Recombinant antibody

SLC25A6-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85368-1-RR
Clone No.242652A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SLC25A6, 242652A8, AAC3, ADP,ATP carrier protein 3, ADP/ATP translocase 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, L02 cells, A549 cells, HuH-7 cells, mouse heart tissue, rat heart tissue, human placenta
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85368-1-RR targets SLC25A6-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
Calculated molecular weight 33 kDa
Observed molecular weight30-33 kDa
GenBank accession numberNM_001636
Gene Symbol SLC25A6
Gene ID (NCBI) 293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12236
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).

Protocols

Product Specific Protocols
WB protocol for SLC25A6-Specific antibody 85368-1-RRDownload protocol
IF protocol for SLC25A6-Specific antibody 85368-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols