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KD/KO Validated

FATP2 Polyclonal antibody

Name: FATP2 Polyclonal antibody
Brand: Proteintech
SKU: 14048-1-AP

FATP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14048-1-AP

Synonyms

SLC27A2, EC:6.2.1.3, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14048-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 14048-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14048-1-AP
IP result of anti-FATP2 (IP:14048-1-AP, 4ug; Detection:14048-1-AP 1:500) with HepG2 cells lysate 3200ug.
IHC staining of human liver cancer using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14048-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (14048-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 14048-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse liver, rat kidney, rat liver, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 36 publications below
IHC	See 4 publications below
IF	See 6 publications below
CoIP	See 1 publications below

Product Information

14048-1-AP targets FATP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FATP2 fusion protein Ag5217 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 27 (fatty acid transporter), member 2
Calculated molecular weight	567 aa, 65 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC057770
Gene symbol	FATP2/SLC27A2
Gene ID (NCBI)	11001
RRID	AB_2239416
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.

Protocols

Product Specific Protocols
WB protocol for FATP2 antibody 14048-1-AP	Download protocol
IHC protocol for FATP2 antibody 14048-1-AP	Download protocol
IF protocol for FATP2 antibody 14048-1-AP	Download protocol
IP protocol for FATP2 antibody 14048-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Hepatology Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding Authors - Wenhao Luo View Article
mouse	WB	Hepatology Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding. Authors - Mallika Mathur View Article
rat	WB	Environ Pollut Hepatotoxicity induced in rats by chronic exposure to F-53B, an emerging replacement of perfluorooctane sulfonate (PFOS) Authors - Xiaohan Li View Article
human	WB	EMBO Rep Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1. Authors - Yang Zhang View Article
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
mouse	WB	Food Funct Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice Authors - Rui Guo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FATP2 Polyclonal antibody

FATP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14048-1-AP

WB analysis using 14048-1-AP

IP experiment of HepG2 using 14048-1-AP

IHC staining of human liver cancer using 14048-1-AP

IHC staining of human liver cancer using 14048-1-AP

IHC staining of human kidney using 14048-1-AP

IHC staining of human kidney using 14048-1-AP

IF Staining of HepG2 using 14048-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding

Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding.

Hepatotoxicity induced in rats by chronic exposure to F-53B, an emerging replacement of perfluorooctane sulfonate (PFOS)

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice