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GLUT1 Recombinant antibody

GLUT1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 81463-1-RR
Clone No.6F14

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

SLC2A1, SLC2A1,GLUT1, GLUT-1, 6F14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inunboiled HEK-293 cells, HeLa cells (incubated at 37°C)
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

For optimal WB detection with 81463-1-RR, we recommend to avoid boiling the sample after lysis.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81463-1-RR targets GLUT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GLUT1 fusion protein Ag16282 相同性解析による交差性が予測される生物種
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Calculated molecular weight 492 aa, 54 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC121804
Gene Symbol GLUT1
Gene ID (NCBI) 6513
RRIDAB_2923718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).

Protocols

Product Specific Protocols
WB protocol for GLUT1 antibody 81463-1-RRDownload protocol
IHC protocol for GLUT1 antibody 81463-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Cancer

Relationship between the expression of PD-L1 and 18F-FDG uptake in pancreatic ductal adenocarcinoma

Authors - Jiajin Li
human,mouseWB,IHC

Cell Death Dis

Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression

Authors - Zhao Cui
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