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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC2A12 Polyclonal antibody

SLC2A12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26958-1-AP

Synonyms

Glucose transporter type 12, GLUT 12, GLUT12, GLUT8, SLC2A12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC2A12 Polyclonal antibody (26958-1-AP)

    WB analysis using 26958-1-AP

    Boiled and unboiled mouse heart tissue were subjected to SDS PAGE followed by western blot with 26958-1-AP (SLC2A12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SLC2A12 Polyclonal antibody (26958-1-AP)

    IHC staining of mouse skeletal muscle using 26958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26958-1-AP (SLC2A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SLC2A12 Polyclonal antibody (26958-1-AP)

    IHC staining of mouse skeletal muscle using 26958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26958-1-AP (SLC2A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse heart tissue, unboiled mouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26958-1-AP targets SLC2A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC2A12 fusion protein Ag24979 相同性解析による交差性が予測される生物種
Full Name solute carrier family 2 (facilitated glucose transporter), member 12
Calculated molecular weight 617 aa, 67 kDa
Observed molecular weight67-70 kDa
GenBank accession numberBC070149
Gene symbol SLC2A12
Gene ID (NCBI) 154091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC2A12 encodes glucose transporter type 12 (GLUT12), a basal and insulin-independent glucose transporter in the heart with previously reported associations with idiopathic dilated cardiomyopathy in humans, as well as insulin resistance and kidney disease in animal models (PMID: 37081215).

Protocols

Product Specific Protocols
WB protocol for SLC2A12 antibody 26958-1-APDownload protocol
IHC protocol for SLC2A12 antibody 26958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols