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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC2A4RG Polyclonal antibody (31429-1-AP)

    WB analysis using 31429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31429-1-AP (SLC2A4RG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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SLC2A4RG Polyclonal antibody

SLC2A4RG Polyclonal Antibody for WB, ELISA
Cat No. 31429-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Huntington disease gene regulatory region-binding protein 1, HDBP-1, HDBP1, HDBP 1, GLUT4 enhancer factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31429-1-AP targets SLC2A4RG in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC2A4RG fusion protein Ag35675 相同性解析による交差性が予測される生物種
Full Name SLC2A4 regulator
Calculated molecular weight41kDa，387aa
Observed molecular weight30 kDa
GenBank accession numberNM_020062.3
Gene Symbol SLC2A4RG
Gene ID (NCBI) 56731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NR83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC2A4RG, or SLC2A4 Regulator, is a nuclear transcription factor that plays a role in activating the solute carrier family 2 member 4 gene, also known as GLUT4. SLC2A4RG has been implicated in the transcriptional regulation of genes, including its interaction with a 7-bp consensus sequence (GCCGGCG), which is an essential cis-regulatory element for Huntington's disease (HD) gene expression in neuronal cells. The SLC2A4RG protein is involved in the modulation of SLC2A4 expression and has been identified as a transcription factor that binds specifically to the promoter region of SLC2A4. It is also known to interact with another transcription factor, myocyte enhancer factor 2 (MEF2), to activate the transcription of SLC2A4. SLC2A4RG is expressed ubiquitously in various tissues, with higher expression levels observed in fat and kidney tissues.

Protocols

Product Specific Protocols
WB protocol for SLC2A4RG antibody 31429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols