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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC30A2 Recombinant antibody

SLC30A2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240405E3

Cat no : 83666-1-RR

Synonyms

ZnT-2, ZNT2, ZnT 2, Proton-coupled zinc antiporter SLC30A2, PP12488

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC30A2 Recombinant antibody (83666-1-RR)

    WB analysis using 83666-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83666-1-RR (SLC30A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using SLC30A2 Recombinant antibody (83666-1-RR)

    FC experiment of HeLa using 83666-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC30A2 Recombinant antibody (83666-1-RR, Clone:240405E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83666-1-RR

    Affinity and Kinetic Characterization of 83666-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83666-1-RR against Human SLC30A2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHaCaT cells, HeLa cells, NCCIT cells, MCF-7 cells, Caco-2 cells, HEK-293 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83666-1-RR is SLC30A2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC30A2 fusion protein Ag8232 相同性解析による交差性が予測される生物種
Full Name solute carrier family 30 (zinc transporter), member 2
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight35 kDa and 52 kDa
GenBank accession numberBC006251
Gene symbol SLC30A2
Gene ID (NCBI) 7780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and function as a homo- or hetero-dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 kDa (short isoform) and 41 kDa (long isoform). This antibody detects 2 bands of 35 kDa and 52 kDa ( glycosylation form of the long isoform) (PMID: 14608047, PMID: 28174721).

Protocols

Product Specific Protocols
WB protocol for SLC30A2 antibody 83666-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols