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SLC30A8/ZNT8-Specific Polyclonal antibody
SLC30A8/ZNT8-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 16169-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse pancreas tissue |
Positive IHC detected in | human pancreas tissue, human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human pancreas tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
16169-1-AP targets SLC30A8/ZNT8-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, rat, Yellow catfish, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | solute carrier family 30 (zinc transporter), member 8 |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | NM_173851 |
Gene symbol | SLC30A8 |
Gene ID (NCBI) | 169026 |
RRID | AB_10733889 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SLC30A8/ZNT8-Specific antibody 16169-1-AP | Download protocol |
IHC protocol for SLC30A8/ZNT8-Specific antibody 16169-1-AP | Download protocol |
IF protocol for SLC30A8/ZNT8-Specific antibody 16169-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes. | ||
J Biol Chem A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells. | ||
J Biol Chem Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8. | ||
J Endocrinol Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death. | ||
Sci Rep A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells. |