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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SLC31A1 Monoclonal antibody (67221-1-Ig)

    WB analysis of HepG2 using 67221-1-Ig (same clone as 67221-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67221-1-Ig (SLC31A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLC31A1 Monoclonal antibody (67221-1-Ig)

    WB analysis of HeLa using 67221-1-Ig (same clone as 67221-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67221-1-Ig (SLC31A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLC31A1 Monoclonal antibody (67221-1-Ig)

    WB analysis of HeLa using 67221-1-Ig (same clone as 67221-1-PBS)

    WB result of SLC31A1 antibody (67221-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells. This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC31A1 Monoclonal antibody (67221-1-Ig)

    IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC31A1 Monoclonal antibody (67221-1-Ig)

    IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC31A1 Monoclonal antibody (67221-1-Ig)

    IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC31A1 Monoclonal antibody (67221-1-Ig)

    IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SLC31A1 Monoclonal antibody (67221-1-Ig)

    IF Staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SLC31A1 Monoclonal antibody (67221-1-Ig)

    IF Staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SLC31A1 Monoclonal antibody, PBS Only

SLC31A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67221-1-PBS
Clone No.1A4H5

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Copper transporter 1, COPT1, CTR1, hCTR1, SLC31A1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67221-1-PBS targets SLC31A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SLC31A1 fusion protein Ag28410 相同性解析による交差性が予測される生物種
Full Name solute carrier family 31 (copper transporters), member 1
Calculated molecular weight 21 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC013611
Gene Symbol SLC31A1
Gene ID (NCBI) 1317
RRIDAB_2882512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15431
Storage Buffer PBS only
Storage ConditionsStore at -80°C.