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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SLC31A1 Recombinant antibody (83844-5-RR)

    WB analysis of HeLa using 83844-5-RR

    WB result of SLC31A1 antibody (83844-5-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

  • Western Blot (WB) analysis of SW480 cells using SLC31A1 Recombinant antibody (83844-5-RR)

    WB analysis of SW480 using 83844-5-RR

    WB result of SLC31A1 antibody (83844-5-RR; 1:800; room temperature for 1.5 hours) with wild-type and SLC31A1 knockout SW480 cells.

  • Western Blot (WB) analysis of various lysates using SLC31A1 Recombinant antibody (83844-5-RR)

    WB analysis using 83844-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83844-5-RR (SLC31A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83844-5-RR

    Affinity and Kinetic Characterization of 83844-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83844-5-RR against Human SLC31A1 were performed. The affinity constant is 3.33 nM.

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  • Featured Product
  • KD/KO Validated

SLC31A1 Recombinant antibody

SLC31A1 Recombinant Antibody for WB, ELISA
Cat No. 83844-5-RR
Clone No.240868D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COPT1, Truncated CTR1 form, CTR1, 240868D10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SW480 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83844-5-RR targets SLC31A1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC31A1 fusion protein Ag26601 相同性解析による交差性が予測される生物種
Full Name solute carrier family 31 (copper transporters), member 1
Calculated molecular weight 21 kDa
Observed molecular weight21-23 kDa
GenBank accession numberBC013611
Gene Symbol SLC31A1
Gene ID (NCBI) 1317
RRIDAB_3671429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC31A1 antibody 83844-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols