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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC31A1 Recombinant antibody (83844-6-RR)

    IF Staining of HepG2 using 83844-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC31A1 antibody (83844-6-RR, Clone: 240868E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using SLC31A1 Recombinant antibody (83844-6-RR)

    FC experiment of HeLa using 83844-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC31A1 Recombinant antibody (83844-6-RR, Clone:240868E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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SLC31A1 Recombinant antibody

SLC31A1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83844-6-RR
Clone No.240868E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

COPT1, Truncated CTR1 form, CTR1, 240868E12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83844-6-RR targets SLC31A1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC31A1 fusion protein Ag26601 相同性解析による交差性が予測される生物種
Full Name solute carrier family 31 (copper transporters), member 1
Calculated molecular weight 21 kDa
GenBank accession numberBC013611
Gene Symbol SLC31A1
Gene ID (NCBI) 1317
RRIDAB_3671430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SLC31A1 antibody 83844-6-RRDownload protocol
FC protocol for SLC31A1 antibody 83844-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols