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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC35A3 Polyclonal antibody

SLC35A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26874-1-AP

Synonyms

Golgi UDP GlcNAc transporter, NGT, SLC35A3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC35A3 Polyclonal antibody (26874-1-AP)

    WB analysis using 26874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26874-1-AP (SLC35A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC35A3 Polyclonal antibody (26874-1-AP)

    WB analysis of HEK-293 using 26874-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26874-1-AP (SLC35A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26874-1-AP targets SLC35A3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC35A3 fusion protein Ag25468 相同性解析による交差性が予測される生物種
Full Name solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member A3
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 32-36 and 41 kDa
GenBank accession numberBC005136
Gene symbol SLC35A3
Gene ID (NCBI) 23443
RRIDAB_2880665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC35A3, also named as UDP-N-acetylglucosamine transporter(NGT), is located to the Golgi apparatus. SLC35A3 can form heterologous complexes with SLC35A2(UGT) and Mgats in the Golgi membrane which may promote biosynthesis of complex N-glycans. It has been reported that the mutation of SLC35A3 is associsted with skeletal dysplasia and epilepsies which could result from impaired glycosylation of proteins. SLC35A3 has some isoforms with 32-36 kDa and 41 kDa.(PMID: 25944901, 28777481  , 16344554, 23583405)

Protocols

Product Specific Protocols
WB protocol for SLC35A3 antibody 26874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Colorectal cancer with low SLC35A3 is associated with immune infiltrates and poor prognosis

Authors - Shuai Lu
View Article