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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC36A3 Monoclonal antibody

SLC36A3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A8E6

Cat no : 67929-1-Ig

Synonyms

FLJ16658, PAT3, SLC36A3, TRAMD2, Tramdorin 2, tramdorin2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SLC36A3 Monoclonal antibody (67929-1-Ig)

    WB analysis of mouse testis using 67929-1-Ig

    unboiled mouse testis tissue were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SLC36A3 Monoclonal antibody (67929-1-Ig)

    WB analysis of COLO 320 using 67929-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC36A3 Monoclonal antibody (67929-1-Ig)

    IHC staining of mouse testis using 67929-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67929-1-Ig (SLC36A3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC36A3 Monoclonal antibody (67929-1-Ig)

    IHC staining of mouse testis using 67929-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67929-1-Ig (SLC36A3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SLC36A3 Monoclonal antibody (67929-1-Ig)

    IF Staining of mouse testis using 67929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SLC36A3 antibody (67929-1-Ig, Clone: 1A8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SLC36A3 Monoclonal antibody (67929-1-Ig)

    IF Staining of mouse testis using 67929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SLC36A3 antibody (67929-1-Ig, Clone: 1A8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inunboiled mouse testis tissue, COLO 320 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67929-1-Ig targets SLC36A3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC36A3 fusion protein Ag16556 相同性解析による交差性が予測される生物種
Full Name solute carrier family 36 (proton/amino acid symporter), member 3
Calculated molecular weight 511 aa, 56 kDa
Observed molecular weight51 kDa
GenBank accession numberBC101092
Gene symbol SLC36A3
Gene ID (NCBI) 285641
RRIDAB_2918681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC36A3 antibody 67929-1-IgDownload protocol
IHC protocol for SLC36A3 antibody 67929-1-IgDownload protocol
IF protocol for SLC36A3 antibody 67929-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols