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SLC38A1 Polyclonal antibody

SLC38A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28632-1-AP

Synonyms

Amino acid transporter A1, ATA1, NAT2, SAT1, SLC38A1, SNAT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC38A1 Polyclonal antibody (28632-1-AP)

    WB analysis using 28632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28632-1-AP (SLC38A1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLC38A1 Polyclonal antibody (28632-1-AP)

    WB analysis of HeLa using 28632-1-AP

    WB result of SLC38A1 antibody (28632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A1 transfected Jurkat cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC38A1 Polyclonal antibody (28632-1-AP)

    IHC staining of mouse brain using 28632-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC38A1 Polyclonal antibody (28632-1-AP)

    IHC staining of mouse brain using 28632-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28632-1-AP targets SLC38A1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC38A1 fusion protein Ag30088 相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 1
Calculated molecular weight 487 aa, 54 kDa
Observed molecular weight54 kDa, 60-70 kDa
GenBank accession numberBC010620
Gene symbol SLC38A1
Gene ID (NCBI) 81539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Protocols

Product Specific Protocols
WB protocol for SLC38A1 antibody 28632-1-APDownload protocol
IHC protocol for SLC38A1 antibody 28632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cell Signal

CENPA promotes glutamine metabolism and tumor progression by up-regulating SLC38A1 in endometrial cancer

Authors - Shuang Li
View Article