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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC38A9 Recombinant antibody

SLC38A9 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240039E7

Cat no : 83182-5-RR

Synonyms

URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using SLC38A9 Recombinant antibody (83182-5-RR)

    WB analysis of mouse pancreas using 83182-5-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83182-5-RR (SLC38A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using SLC38A9 Recombinant antibody (83182-5-RR)

    FC experiment of U2OS using 83182-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Slc38a9 Recombinant Antibody (83182-5-RR, Clone:240039E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83182-5-RR

    Affinity and Kinetic Characterization of 83182-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83182-5-RR against Human SLC38A9 were performed. The affinity constant is 0.715 nM.

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Tested Applications

Positive WB detected inmouse pancreas tissue
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83182-5-RR targets SLC38A9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC38A9 fusion protein Ag15607 相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 9
Calculated molecular weight 561 aa, 64 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC066891
Gene symbol SLC38A9
Gene ID (NCBI) 153129
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that responds to multiple environmental cues. The lysosomal amino acid transporter SLC38A9 is a physical and functional component of this sensing complex. SLC38A9 (Solute Carrier Family 38 Member 9) was first identified as a potential amino acid transporter belonging to the slc38 family. SLC38A9 has some isoforms: 64,53,57 kDa.

Protocols

Product Specific Protocols
WB protocol for SLC38A9 antibody 83182-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols