Various lysates were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

LNCaP cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

Jurkat cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

K-562 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.